Effects of Pomegranate Peel Polyphenols(PPPs) on the Expression of HMG-CoA Reductase mRNA of Human L-02 Hepatocyte

Abstract:

The objective of this manuscript is to study the effects of Pomegranate Peel Polyphenols,Punicalagine and ellagic acid on the expression of HMG-CoA reductase mRNA of human L-02hepatocyte. The model of steatotic L-02 hepatocyte was firstly established by cultured with 50%(V/V) fetal bovine serum for 48 hours,and the appropriate concentrations of PPPs were detected by MTT assay. Then the experiments were divided into three groups:normal group、model group and therapy group,different contents of PPPs is added to the therapy group. After 48 h cultured,the mRNA expression level of HMG-CoA reductase was detected by RT-PCR after 48 h cultured. It was found that the HMG-CoA reductase mRNA expression level was significantly inhibited by PPPs treatment groups,especially for the case of 100 μg/mL Punicalagin group. From the aboved results,the possible mechanisms of PPPs decreasing the intracellur cholestol are related to downregulation of HMG-CoA reductase mRNA’s expression,Punicalagin was the key component in PPPs on lipid-lowering.