Vasodilating Effect of BNP in Different Kind of Buffer Systems

Abstract:

To investigate the vasodilating effect of BNP in different buffer solutions,rabbit thoracic aorta was rapidly dissected out and the effect of BNP on the tension of aortic rings was measured in an organ bath system. The results showed that BNP in Citric acid/Na2HPO4 buffer caused a concentration-dependent relaxation. However,BNP in NaAc/HAC or K2HPO4/KH2PO4 buffer has vasodilating effect in dose-independent manner. The results indicate that BNP in Citric acid/Na2HPO4 buffer could keep its vasodilating effect in a dose-dependent manner,implying that they may be effective in BNP formulation.