Study on Anti-Avian Leukosis Virus Subgroup A Activity of Anthocyanin from A Blackcurrant

Abstract:

Inhibitory action of anthocyanin from blackcurrant(ACB) to avian leukosis virus subgroup A(ALV-A) were studied.ACB powder was chosen as the raw material and its anthocyanins structure was identified by HPLC-MS.The morphology feature and survival rate of cells treated with anthocyanin were determinated by using MTT method and microscope.Furthermore,establishing the cell modle of anthocyanins inhibiting ALV-A which infecting in DF-1 cells,and then researched ACB’s prevention and treatment roles on the cells which infected with ALV-A.The results showed that ACB contained two main components as delphinidin-3-rhamnoside-5-glucoside and cyaniding-3-rhamnoside-5-glucoside.The cells grew well that treated with anthocyanin under the concentration of 10 μg/mL.There was a significantly inhibitory effect on restraining the proliferation of ALV-A after treating with anthocyanins.It was concluded that ACB had an inhibitory effect on ALV-A in vitro.