Assessment of β-D-Glucosidase Activity of Oenococcus oeni 31MBR

Abstract:

β-D-glucosidase activity of Oenococcus oeni 31MBR was assayed spectrophotometrically with synthetic substrate p-nitrophenyl β-D-glucopyranoside.Results showed that Oenococcus oeni 31MBR exhibited considerable β-D-glucosidase activity,which was mainly intracellular form and soluble enzyme.Enzyme activity was highest at the mid-log phase,and showed a downtrend after this period.β-D-glucosidase of Oenococcus oeni 31MBR was constitutive,however,increased enzyme activities were observed when induced with both arbutin and cellobiose.Oenococcus oeni 31MBR proved potential for aroma enhancement during malolactic fermentation,this is significant for winemaking.