Preparation and Evaluation of Melamine Immunoaffinity Column

Abstract:

An immunoaffinity column(IAC)for melamine with high column capacity was prepared in this study.Based on a monoclonal antibody against melamine,a silanization approach was used to coat antibody as ligands.The resulting products were packed in columns.Series concentration of melamine was loaded on the columns to evaluate column’s performances.UV spectrum and liquid chromatography tandem mass spectrum(LC-MS) were used to test the melamine level of eluents from columns.The mean load capacity of the prepared columns was 16.47 μg per column.Under the optimized conditions(washing condition,loading buffer,eluent condition and regeneration buffer),the proposed IAC has a condensation coefficient of 66 folds and it could be used over 5 times with the recovery higher than 60%.