Study on the Effect of Protective Additives on Stability Mechanism of Cholesterol Oxidase

Abstract:

An effective method to keep the stability of cholesterol oxidase(COD) was provided by studying on stability mechanism of COD.Deactivation mechanism of COD was analyzed by fluorescence spectra,circular dichroism spectra and SDS-PAGE.We added two additives into the preparation of COD and stabilization mechanism was studied.The results indicated that 15% glycerin was selected as the best protector at 37 ℃ and the remained enzyme activity was increased by 58% after 45 hours.Riboflavin had no significant effect on enzyme stability.A mechanism for the glycerin effect on inhibiting protein aggregation,protecting protein secondary structure and maintaining high activity was proposed.It also showed that riboflavin had no protective effects against protein aggregation.