Medium Optimization for the Production of β-1,3-1,4-Glucanase by Bacillus tequilensis CGX5-1

Abstract:

Bacillus tequilensis CGX5-1 is a newly screened strain which can effectively secrete β1,3-1,4-glucanase.Fractional factorial design was used to clarify the medium components and the concentration of barley flour and soy bean flour were found to be the key factors.The steepest ascent experiments was applied to determine the optimal domain and the central composite design was used to estimate the quadratic response surface,thus we acquire the factor values for maximum production of β-1,3-1,4-glucanase.The final composition of fermentation medium was determined via response surface methodology,which was(g/L):barley flour 68.4,corn flour 40,soybean flour 61.1,KH2 PO4 1,MgSO4 ·7H2 O 0.1,CaCl2 0.1.The highest activity of β-1,3-1,4-glucanase was 191.96 U/mL at 52 h culture using optimized medium,which was 1.91 times higher than that from original medium.