Abstract:The aims of this manuscript were to study arsenic speciation and content in kelp with different processing methods(direct drying,blanching,blanching-salting-washing) by high performance liquid chromatography coupled to inductively coupled plasma-mass spectrometry(HPLC-ICP-MS).The results showed that the total arsenic content in direct drying kelp was 47.23 mg/kg and blanching kelp was 42.91 mg/kg(dry weight) with no significant differences(P>0.05).The total arsenic content in blanching-salting-washing kelp was 25.62 mg/kg(dry weight),there was extremely significant differences(P<0.01) from direct drying kelp and blanching kelp.The total arsenic content in kelps was gradually decreased after blanching-salting-washing,while the saltingwashing process decreased most obviously.Arsenocholine,arsenobetaine,dimethylarsinic acid and three substances suspected as arsenicsugar were the major arsenic forms in kelps,which arsenicsugar was in the hightest amount(w>95%) and inorganic arsenic was in the lowest amount(w<1%).