Abstract:Effect of the aqueous fraction from ethanolic extract of Boschniakia rossica(BRAF) on the oxidative stress and apoptosis of liver in mice with an acute liver injury induced by Lipopolysaccharide(LPS) and D-galactosamine(GalN) was investigated in this manuscript.The mice were randomly assigned to 5 groups: normal control,model control,silymarin(positive control),as well as high and low dose groups of BRAF.Animals were treated once daily for 7 days.At the end of the experiment,LPS and GalN were given intraperitoneally to the mice of groups,then the pathological changes was detected with hematoxylin & eosin(HE) staining,tumor necrosis factor-α(TNF-α) was detected by ELISA method,the alanine aminotransferase(ALT),aspartate aminotransferase(AST),catalase(CAT),glutathione peroxidase(GPx),glutathione S-transferase(GST),reduced glutathione(GSH),malondialdehyde(MDA) and nitric oxide(NO) were assayed with colorimetric method,and DNA damage was analyzed using the electrophoretic method.The results showed that the administration with BRAF reduced the serum ALT and AST,alleviated the hepatic lesions induced by GalN/LPS,decreased serum TNF-α level,reduced the hepatic NO and MDA contents,increased the hepatic CAT,GPx,GST and GSH levels,and reduced the DNA damage of hepatocyte in mice with acute liver injury.It is suggested that BRAF has a protective effect on acute liver injury induced by GalN and LPS in mice,probably via antioxidative and anti-apoptotic abilities.
