Research Progress in Separation and Purification Methods of Antifreeze Proteins from Plants

Abstract:

Antifreeze proteins are the thermal hysteresis proteins,which have the ability to modify ice growth and inhibit ice recrystallization.Antifreeze proteins from plants are of significance to elongate shelf-life of frozen food and improve their quality.How to separate antifreeze proteins from complicated extract liquids effectively has been a hot and challenging topic.In this article,the recent advances in the separation and purification methods of antifreeze proteins from plants are reviewed and the basic principles of the methods are introduced.The methods include traditional chromatography separation,SDS-PAGE electrophoresis,ice-affinity adsorption and cloud point extraction.Based on the above research progress,the future directions to develop separation methods for antifreeze proteins are put forward.