Study on Pretreatment Processing of Gelatin Extraction from Tilapia Bones

Abstract:

This paper is designed to extract gelatin from tilapia bone with the method of Enzymatic Hydrolysis.The effect of demineralizing and hydrolyzing conditions on extraction were analysed.In this research we can show the demineralizing condition is tilapia bone added to 0.6mol/L HCl with solution-to-bone ratio 5 mL/g for 6 h and change the acid solution every 1.5 h.the optimal conditions of enzymolysis is the temperature 46.5 ℃,pH 5.3,time72 min,the protease solution-tobone ratio was 3 mL/g,amount of enzyme 2480 U/g(bones).