Rapid Differentiation and Identification of Three Species of Listeria by FT-IR Spectroscopy

Abstract:

To establish a standard fourier transform infrared(FT-IR) spectral library and a FT-IR method of differentiation and identification of three species of Listeria,viz Listeria monocytogenes,Listeria iuanuii and Listeria innocua,FT-IR fingerprint absorption spectra of three species of Listeria were collected,and a standard spectral derivatives library was created.Combined with chemometrics methods,two cluster models of principal component analysis(PCA) and hierarchical cluster analysis(HCA) were established.It was found that three species of Listeria could be well differentiated and identified,and the standard spectral derivatives library created could be used to compare with those of target Listeria and identify them.As a rapid,easy-to-use and accurate technique,FT-IR spectroscopy is an effective tool to differentiate and identify three species of Listeria studied here.