Abstract:Maltotetraose is a key sugar in maltooligosaccharide family.It has quite some unique physical and chemical properties,such as low sweetness,low osmotic pressure,high viscosity,acid resistant and so on,which results in its wide application in food industry.Maltotetraose-forming amylases have been reported before,but few of them could be industrialized.The less thermostability and acid stability of the enzyme properties,and the cost of the enzyme make commercial production of the sugar less viable.A new acid stable and thermostable maltotetrasoe-forming amylase is introduced in this paper,and its operational process is optimized.The results showed that this new amylase could be operated at pH4.0~8.0,temperature from 55~70 ℃.At the same time,more than 50% maltotetraose syrup could be obtained with the presence of pullulanase.The new enzyme makes the production of maltotetraose more competitive.
