Mathematical Modeling for the Optimum Enzymatic Hydrolysis of Rana Skin Collagen Peptide and Its Antioxidant Research

Abstract:

The influencing factors in the enzyme solution process of Rana skin collagen peptide(LWT) were optimized by using the mathematical statistics and data fitting analysis method.And the relationship between the influencing factors and hydrolyzing degree in the enzyme solution process of LWT was established.And the best value hydrolysis degree(peak) was forecasted.Used polyacrylamide gel electrophoresis method to determine the molecular weight of LWT which was under 3 500.High performance liquid chromatogram showed that the purity was in the high level.In the antioxidant effect of LWT on liver damage of bromobenzene mice were studied.The results showed that LWT have the remarkable effect on reducing MDA content and the obvious effect on rising SOD vitality in mice liver tissue and have antioxidant effect.