Abstract:The conditions of extraction and separation and the dissolution characteristics on the pigment of fusarium are studied.The results of Experimental show that the best extraction solvent of the pigment is pure acetic.The pigment only can dissolve in organic acids and acidic organic solvents.The best high-performance liquid separation conditions of the pigment are the followings:the column is Phecda C18(4.6 mm×250 mm,5 μm).The temperature of the column is 25 ℃.The detection wavelength of the DAD detector is 254 nm.The flow rate is 1.0 mL/min.The injection volume is 1μL.The mobile phase A is acetonitrile containing one-thousandth of trifluoroacetic acid.The mobile phase B is distilled water containing one-thousandth of trifluoroacetic acid.The gradient elution program is that:mobile phase A,0~3 min,2%~6%;3~3.1 min,6%~45%;3.1~28 min,45%~70%;28~32 min,70%~80%.Eight kinds of pigments are obtained,and four kinds of pigments are red,three orange,one yellow in acidic acetonitrile.In accordance with the order of the peaks,their maximum absorption peaks are 508、458、456、498、510、456、454、506 nm respectively.
