Cloning and Expression of Benzoylformate Decarboxylase Gene and Study on Biotransformation of Ethyl Vanillin by Resting Cell

Abstract:

Benzoylformate decarboxylase gene(mdlC) from Pseudomonas putida ATCC12633 was inverted into Escherichia coli(E.coli) strain BL21(DE3) and was efficiently expressed after induction with IPTG.The recombinant strain together with E.coli/pET30a-mdlB converted successfully(S)-4-hydroxy-3-ethoxymandelic acid(EMA) to ethyl vanillin in the forms of mixed resting cells.Without optimization,all the(S)-EMA was consumed to form ethyl vanillin(1.94 g/L) and no by product was obtained with the initial substrate concentration 5 g/L by after 24 h.The cells could maintain their enzyme activity in repeated utilization at least three times and shortened bioconversion time efficiently.