Isolation and Identification of the Alkaline Protease-Producing Bacteria and Optimization of Its Culture Conditions

Abstract:

Research of screening and fermentation about Bacillus sp.as the protease producing strain is extremely necessary due to their characteristics of heat and pH stability.In this study,strain with producing alkaline protease was screened from the offshore soil of Yantai and the morphological,physiological and biochemical characteristics as well as 16S rDNA sequence homology of the isolation were studied.From the above characteristics,the strain was identified as Bacillus amyloliquefaciens.For industrial production of protease,the optimum nutrition and environmental conditions were determined.Under the optimum conditions,the maximum protease activity reached at 395.18 U/mL at 18 h,which was about 2.55 fold higher than that of the the original medium.The study provides reference data for further industrial fermentation production of alkaline protease.