Authentication of Shark Derived Material in Food Using SYBR Green Fluorescence Real-Time PCR

Abstract:

A specific and sensitive SYBR Green real-time fluorescence PCR method was developed to determine of the shark derived material in food.The specificity and sentivity of this method was examined.The results showed 9 kinds of shark samples displayed theamplified curves and the highest point of melt curves at(84±1.5) ℃,whereas other 48 kinds of common plants and animals displayed no amplified curves and the highest point of melt curves at temperatures out of(84±1.5) ℃.The detection limit of the method was 0.000 1 ng/μL DNA and 0.01% shark meat powder.The 20 commercial shark products samples were analysized by SYBR Green PCR and all the products indicated existence of shark DNA except the artifical shark fin and shark liver oil.The results indicated that the detection method is highly specific and sensitive,and can be used to the identification of shark derived material in food.