Abstract:The objective of this study was to investigate the diversity of culturable yeast strains from permafrost soils at the terminus of a glacier in the Tianshan Mountains was investigated.Isolation and molecular phylogenetic analysis were performed in order to expand our knowledge on diversity of psychrotrophic and psychrophilic yeast.In addition,efforts focused on screening for psychrophilic yeast.Psychrophilic yeast strains were detected on YEPG plates,respectively.Identity and genetic diversity of strains isolated was determined by 26S rRNA gene D1/D2 by the forward primers NL1 and the reverse NL4.The physiological tests were carried out to determine the phonotypic differences between strains showing high similarity of 26S rRNA gene sequences.In all,4 yeast stains was isolated by 26S rRNA gene D1/D2 and internal transcribed spacers of 4 genera(Cryptococcus,Sporobolomyces,Clavispora,Pichia).The results enrich our knowledge on the differences of psychrotrophic yeast diversity between the glacier under study and other worldwide cold habitats are also discussed.
