Study of Trametes hirsuta SYBC-L19 Liquidstate Fermentation for Laccase Production

Abstract:

In this study,the nutritional and environmental conditions of white-rot fungi,Trametes hirsuta SYBC-L19,for laccase production were investigated and optimized though single factor experiments and response surface methodology.The optimum conditions described as follows:water hyacinth(dry weight) 41.0 g/L,corn steep powder 50.8 g/L,glucose 30.0 g/L,CuSO4·5H2O 0.6 g/L,tween-80 0.5 g/L,vanilline 0.5 mmol/L,inorganic salt mixture 50 mL/L,inoculation concentration 8%,pH 4.0,liquid volume 80 mL/250 mL,culture temperature 30 ℃.With the conditions,the activity of laccase was achieved at 7 784 U/L,was higher 17.3 times than that of control.The study provides a path and a certain theoretical basis for effective utilization of the water hyacinth and other biomass resource.