Optimization of the Extraction Technique and the Antioxidant Activity of Polysaccharides from Pleurotus geesteranus

Abstract:

The ultrasonic extraction process of polysaccharides from Pleurotus geesteranus was optimized by response surface analysis,and the antioxidant activity of the extracts were determined.The results showed that,the optimum ultrasonic extraction conditions were obtained as follows:temperature at 40 ℃,ultrasonic power at 400 W,time duration for 114 min,and the ratio of solution(water) to solid(fruit body powder) of 30∶1.Under this condition,the extraction yield of the polysaccharide from Pleurotus geesteranus was 60.19 mg/g,which was consistent with the predicted value(60.74 mg/g).The Polysaccharide extracts have a certain extent function to scavenging hydroxyl radical.Their antioxidant power and the reducing capacity increased with the increase of polysaccharides concentration.