Screening and Identification a Serratia marcescen Strain Producing Red-Pigment and Preliminary Study of the Fermentation Conditions

Abstract:

A strain producing red-pigment was newly isolated under poor nutrition conditions.It was identified by 16S rDNA sequence and systematic analysis,as well as general morphological and biochemical characteristics,The results show that 16S rDNA sequence of the strain suggesting that the strain is Serratia marcescens Species.And it was named Serratia marcescens JNB5-1.The red-pigment was identified as prodigiosin by wavelength scanning and LC-MS.preliminary fermentation conditions of strain Serratia marcescens JNB5-1 was studied.The results showed that the yield of prodigiosin was improved to 4.139 g/L after 72 h in the medium:sucrose 2%,beef extract 1.5%,CaCl2 1%,proline 0.75%,MgSO4·7H2O 0.02%,FeSO4·7H2O 0.006%.