Enrichment,Purification and Determination of Sterol Glycosides in Biodiesel

Abstract:

The method for enrichment,purification and determination of glycosides in biodiesel by GC was developed in this manuscript.Sterol glycosides was purified and enriched by saponification,liquid-liquid extraction,SPE(Solid Phase Extraction),and quantified using β-cholestanol as internal standard by GC.The different pre-treatments methods for biodiesel,emulsified biodiesel and biodiesel residue and effect of strength of saponification on determination of sterol glycosides were discussed.The results showed that: biodiesel was treated without saponification,and the optimum conditions for emulsified biodiesel and biodiesel residue listed as follows: that t 0.1mol/L KOH-ethanol solution,60℃saponifying temperature,40min saponifying time.The results suggested that were helpful to improve the control of biodiesel quality and the utilization of by-products in biodiesel.