Isolation and Identification of Cholesterol-Reducing Lactic Acid Bacteria from Pig

Abstract:

Lactic acid bacteria are a kind of important probiotics,which have the ability of cholesterol-reducing.Total 31 strains of Lactic acid bacteria were isolated from pig source.Among these strains,18 strains could grow in the medium containing high cholesterol using phosphate pyrite method and the cholesterol-removal rate of those strain varified from 0 to 47.26% in vitro.Among them,four strains,namely r16,r53,r762,and m66,exhibit high cholesterol reducing ability more than 30%,.Based on the morphological,physiological,biochemistry characteristics and 16S rDNA sequencing analysis,strain r16,r53,and r762 were identified as Lactobacillus plantarum,and m661 as Enterococcus gallinarum.