Fermentation and Extraction of Polysaccharides from Coriolus versicolor and Its Antioxidant Activity

Abstract:

By using single factor experiment and central composite design,the optimal culture conditions of Coriolus versicolor for polysaccharides production were determined as polyptone 5.870 g/L,maltose53.120 g/L,pH 5,temperature 28℃ shear speed 150 r/min.Under these conditions,the EPS production reached at 10.0982 g/L.Furthermore,EPS were treated by Sevag method to remove the protein and purified by gel filtration on Sepharose CL-6B chromatography.The antioxidant activity was determined by Phenanthroline-Fe2+ method and DPPH assay.The polysaccharides showed notable inhibitory activity of OH and DPPH radical.Thus,the EPS from C.versicolor may be supplied as one natural healthy food.