Abstract:The objective of this study is to investigate the optimization of extraction conditions of antibacterial substance from Kadsura longipedunculata and antimicrobial activity.Spoilage bacteria and funguses were used as the tested strains.According to the single-factor experiments,four levels of the factors were selected for the central composite design and response surface methodology was employed to optimize the extraction conditions of from K.longipedunculata.And the antimicrobial activity of K.longipedunculata extract was investigated.The optimum extraction conditions were as follows: concentration ethanol of 50%,extraction pH of 6.76,liquid-to-solid ratio of 18∶1(mL/g),temperature of 75 ℃and time of 3.9 h.The diameter of inhibition zone of extracts against Staphylococcus aureus reached with verified value being of 27.5mm that was almost equal to the estimated value being of 27.68 mm(CI>95%).The extract had the prominent antibacterial activity on bacteria and the antimicrobial diameters were greater than 20mm.While there was no sensitivity against Colletotrichum magiferae and Aspergillus niger,et al.The MICs on test bacteria were 3.125~25mg/mL.
