Effect of Macronutrient on Growth and Polysaccharide Content of Cyclocarya paliurus Suspension Cells

Abstract:

In order to provide reference for production of Cyclocarya paliurus polysccharide on a large scale in bioreactor,and solve the problem of Cyclocarya paliurus resources scarcity,the effects of nutrition conditions on on the growth and polysaccharide content of C.paliurus suspension culture cells were studied in this manuscript.The single factor experiment and orthogonal test were applied to optimize the nutrition conditions and the result were listed as follows: for cell growth: 40 g/L sucrose+30 mmol/L total nitrogen +2 mmol/L Mg2++4 mmol/L Ca2+.for polysaccharide production:40 g/L sucrose+30 mmol/L total nitrogen +3 mmol/L Mg2++3 mmol/L Ca2+.