Study on Filming Performance of Wool Keratin with the Catalysis of Transglutaminase

Abstract:

In this study,transglutaminase was adopted to catalyze wool keratin cross-linked and the effect of reaction conditions such as enzyme dosage,pH value of film-forming solution, temperature,treatment time on the mechanical properties of the film were determined.In addition, the effect of enzymatic cross-linking reaction on the stability of the wool keratin film in hot water was investigated.The keratin protein molecular change induced by TGase-mediated cross-linking was analyzed by SDS-PAGE.The results showed that,TGase-catalyzed preparation of keratin film could increase tensile strength and stability of water,but decrease the elongation. The optimum conditions were as follows:30 U/g wool keratin of TGase,40℃,pH 7.5 of film-forming solutions,18 h of treatment time.SDS-PAGE result demonstrated that new biopolymers with higher molecular weight from wool keratin were formed by using TGase.