Effects of External Hormone on Inducing Perilla Frutescens Callus and Accumulation of Rosmarinic Acid

Abstract:

In this study,with the leaves of Perilla frutescens were used as explant,effect of different concentrations of the hormone combination on inducing callus in B5 medium were studied by orthogonal test.It was found that the optimum medium for callus induction is B5 + 2,4- D1.0 mg/L+KT0.4 mg/L+6-BA1.0 mg/L+NAA1.5 mg/L,under the optimum conditions, the highest induction rate was achieved.Furthermore,the content of rosmarinic acid in calli after 40 days culture was achieved at 3.85%,however,the value in the leaves of Perilla frutescens was only 0.25%.The results here provided a theoretical basis for the establishment of high-yielding cell lines.