Molecular Identification of Nine Strains Lactic Acid Bacteria Isolated from Yak Milk and Triton in Sichuan

Abstract:

In this study,To accurately identify lactic acid bacteria(LAB) that were isolated from fresh yak milk and Qula in Sichuan region of China,nine LAB strains were classified and identified to species level by using 16S rDNA sequencing analysis,multiplex PCR assay of recA gene,hsp60-PCR-RFLP analysis etc.The results showed that nine LAB strains were initially characterized as Lactobacillus plantarum group(4 strains),Leuconostoc mesenteroides(4 strains) and Lactobacillus helveticus(1 strain) by 16S rDNA sequencing analysis.However,16S rDNA sequencing analysis could not distinguish between L.plantarum and L.pentosus.For further identification of strains in L.plantarum group,multiplex PCR assay of recA gene and hsp60-RFLP analysis were conducted.The results revealed that multiplex PCR assay of recA gene and hsp60-RFLP could identify L.plantarum group to species level and 4 LAB strains in L.plantarum group were identified as L.plantarum.