Study on the Seperation,Purification and Some Properties of the Protease from the Fruits of Siraitia grosvenorii

Abstract:

The structure-function relationship of the protease from the fruits of siraitia grosvenorii was studied by analyzing the effects of modification on the protease activity.The activities were detected after the protease was modified by 8 kinds of protein modification reagents,respectively.And the results showed that the enzyme activity was significantly decreased after modified by PMSF,NBS and DEPC,but the others did not had obviously effected.So the hydroxyl group,indolyl group and imidazole group were demonstrated to be essential to the enzyme’s function probably,but the sulfhydryl group,amino group,garboxyl group,guanidino group and disulfide may not be essential for the activity of the enzyme.