Establishment of the Direct Competition ELISA Method for Deoxynivalenol(DON)

Abstract:

In this manuscript,the direct competition ELISA method for deoxynivalenol(DON) was established by using enzyme-labeled antigen of DON and antibody.It was found that thedetection range of this method is 1 ～ 100 ng/mL,sensitivity 0.56 ng/mL,50 % inhibitory concentration(IC50) 10 ng/mL,the cross-reaction rate to DON analogues T-2 toxin 12%,the recoveries of corn starch samples 80.2%～91.1%.Moreover,the average intra-assay variation and the average inter-assay variation is less than 15%,and 3%,respectively.