Isolation and Characterization a Strain for Bioconversion Anethole to Anisaldehyde

Abstract:

Using trans-anethole as the sole carbon source,a strain WGB30 was screened from 320 isolates,and 50 environmental samples by thin-layer chromatography,high efficiency liquid chromatography and spectrophotometry.It was found that the optimum temperature and pH is 28 ℃ and 6.5 for this strain,respectively.One loopful of the plate culture was inoculated into 100 mL medium containing 0.5% anethole as carbon source incubated at 30 ℃ for 60 h with reciprocal shaking(200 strocks/min),the ratio conversion of anisaldehyde is up to 5.08%.According to the morphological features,the results of physiological and biochemical experiments and 16S rDNA sequence analysis,this strain was identified as Burkholderia sp.