Effect of Soapberry Extraction on Lipase Activity of Fusarium sp. and Lipase Extraction by Aqueous Two-Phase Systems

Abstract:

Effect of surfactant on lipase production of Fusarium sp.was investigated in this manuscript.The results showed that lipase activity was achieved at the maximum value in adding soapberry extraction,which indicated that soapberry extraction is the best surfactant,and lipase activity was the highest when the liquid-to-solid ratio is 6∶1(mL∶g).Lipase extraction by Aqueous Two-phase System following the precipitation of the crude enzymes by ammonium sulfate was also studied.The results indicated that the highest of decontamination factor F and extraction rate Sf achieved when the conditions of extraction showed as follows:the molecular weight of PEG was 4000,the concentration of PEG(M 4000)was 30 g/dL,and the concentration of NaH2PO4 was 20 g/dL.At last,the lipase was purified 7.92 folds and the recovery was 48.30% by Aqueous Two-phase Systems.