Optimization of Shiraia bambusicola Liquid-State Fermentation for Laccase Production

Abstract:

A new laccase producing strain,Shiraia bambusicola,was isolated from the natural screening.In this study,the optimum nutrition and environmental conditions were investigated and listed as follows: f soluble starch 2%,yeast extract 0.8%,and copper ion 2.4 mmol/L,pH 5.0,liquid volume 50/250 mL,30 ℃,200 r/min.With the optimum conditions,the activity of laccase was achieved at 120 000 U/L after 64 h,was higher 17 times than that of the control.Compared with the reported white-rot,Shiraia bambusicola has the advantages of short fermentation period and higher laccase activity.