Optimization of Medium of Trichoderma viride Using Response Surface Methodology

Abstract:

In this study,response surface methodology(RSM) was used to optimize the medium compositions for Trichoderma viride growth with the colony growth diameter of T.viride as index.According to the single-factor experiments,three levels of the factors(carbon source addition,nitrogen source addition and phosphorus source addition) were selected for the central composite design to establish a quadric regression equation,and RSM was employed to optimize.The results showed that the optimal conditions for Trichoderma viride were 2.11% glucose,0.15% alanine and 0.3% KH2PO4.Under these conditions,the colony growth diameter of T.viride reached at 78.00 mm,which t was almost equal to the estimated value 77.87mm(CI>95%).