Molecular Cloning and Sequence Analysis of Geranyhlgerany Pyrophosphate Systhase from Taxus wallichiana var. Mairei

Abstract:

In this study,the DNA sequence and cDNA sequence of ggpps were cloned from Taxus wallichiana var.Mairei.Results showed that the length of both DNA and cDNA sequences is 1182 bp,without introns,contained an opening reading frame of 1182 bp,which coded for 393 amino acid residues;theoretical molecular weight is 42.59kDa,and isoelectric point is 5.68.Alignment showed that cDNA sequence exhibited 98.8 % similarity to the ggpps gene of Taxus canadensis(AF081514) which has been reported in NCBI,and its protein sequence has 99.0 % similarity to GGPPS of Taxus canadensis(AAD16018).The bioinformatics of GGPPS,including physical and chemical properties,the signal peptide,hydrophobicity,hydrophilicity,secondary structure,tertiary structure,and phylogenetic trees,were analyzed with bioinformatics softwares and database,such as Protparam,SignalP,ProtScale,SOPMA,Swiss-Modeling,Scan Prosite,MEGA4.0 and so on.