Distribution and Characterization of Vibrio parahaemolyticus in Shellfish from Eastern Coast of China

Abstract:

To investigate the distribution,serological and molecular characterization of vibrio parahaemolyticus of shellfish,a total of 288 samples were collected and analyzed in this manuscript.The levels of V.parahaemolyticus were estimated by MPN-PCR and isolated strains were clustered by RAPD analysis.The tdh and trh genes were detected by PCR.The results showed that the distribution of V.parahaemolyticus was disparity in season,the level of V.parahaemolyticus in summer were different from other seasons(P<0.05).Altogether 172 strains were isolated and identified,of which 157 strains were typed by O-grouping serum and 42 strains were typed by K-typing serum,the group of O3(19.1%) was the most frequently involved in shellfish.RAPD analysis showed that there were 73 different patterns which were clustered into 18 groups.Two isolates showed positive of tdh gene and 5 isolates showed positive of trh gene,there was no strain with tdh and trh genes simultaneously.The serovars of two isolates with tdh gene were O3∶K6 and O4∶K68 and two isolates were grouped into the same cluster by RAPD analysis.