Enzyme Characterization of a New Strain of Chitinase

Abstract:

Chitinase from a new isolated strain Chitinolyticbacter meiyuanensis SYBC-H1 was purified and characterized in this study.Chitinase of more than 95% purity was obtained after ammonium sulfate precipitation,DEAE-32 negative chromatography and Sephadex G-100 sieve chromatography.The results of enzyme properties indicated that its optimal pH and temperature was 40℃ and 6.5,respectively.Chitinase exposed to temperature lower than 50℃ was stable and it is most stable at pH 5.0.Its stability was promoted with the addition of mercaptoethanol and EDTA.Moreover,the chitinase activity was apparently stimulated by Na+ and K+,while inhibited by Zn2+,Mn2+,Fe2+ and Fe3+.The shrimp crust was hydrolyzed into acetylglucosamine by Chitinase from SYBC-H1,and the hydrolysis rate achieved at 21.8%.