Effect of Different Components of Bacillus megaterium on Gluconobacter oxydansin Mix-Cultured of Vitamin C Fermentation

Abstract:

In the process of vitamin C two-step fermentation,Bacillus megaterium(B.m) can enhance the ability of Gluconobacter oxydans(G.o) in convertingL-sorbose into 2-Keto-L-gulonic acid(2-KLG).In this study,isolated and purified active proteins were conducted by salting out of using ammonium sulfate fractionation,column chromatographyand electrophoresis technologyfrom the supernatant of B.megaterium.The effects of different components from the supernatant of B.megaterium on Gluconobacter oxydans growth and the ability of itsL-sorbose dehydrogenaseand yield of 2-KLGwere investigated,respectively.Among which the separated metabolites with molecularweightof 36 000 and 44 300 from B.megaterium significantly enhance the G.oxydansgrowth and 2-KLG production.