Research of Preparation of Casein Bioactive Peptides Using Enzymatic Membrane Reactor

Abstract:

The influence of operating conditions including pressure and recirculation flow rate on the isolation of casein bioactive peptides was investigated in this study.Alcalase 2.4 FG was used to continuously hydrolyse an initial concentration of 6%(w/w) casein at pH 8.5 and 50 ℃ in an enzymatic membrane reactor.The degree of hydrolysis(DH),permeate flux(J),product recovered in permeate(S),enzyme leakage(AF),residual enzyme activity(AR) and enzyme loss(AL) were determined to value the effect of operating conditions of pressure and recirculation flow rate on isolation of casein bioactive peptides by single factor experimental.It showed that a high increased in S with increasing operating pressure,but leading to a serious enzyme leakage and membrane fouling.However,it could reduce enzyme leakage and membrane fouling with a suitable recirculation flow rate of 140 mL/min,meanwhile it could gain a relatively high protein recovery.