Optimization of Extraction Process by Using Response Surface Methodology and Antioxidant Activity of Polysaccharide from Yellow Mealworm

Abstract:

In this study,respone surface analysis methodology(RSM) was applied to optimize the extraction conditions of polysaccharide from yellow mealworm(Terebrio molitor L.).Antioxidant capability of the polysaccharide was studied in vitro.The optimal range of key factors were studied by regression analysis and listed as follows: temperature 90 ℃,time 158 min,the ratio of liquid to solid 12.With the optimum conditions,the yield of polysaccharide was achieved at 1.94%(the predicted yield was 1.99%).The polysaccharide from yellow mealworm was constituted glucose mainly,mannose and galactose.The results also demonstrated that yellow mealworm polysaccharide has antioxidant activity in vitro,which means it could be explored as a potential natural antioxidant.