Studies on Purification of Cordycepin from Fermented Broth by Macroporous Resin

Abstract:

In this study,macroporous resin was used to extract cordycepin from fermentation broth of Cordyceps militaris.By compared with the adsorption and desorption effects of six kinds of macroporous resins to cordycepin,NKA-Ⅱ macroporous resin exhibited the excellent characteristics for cordycepin purification.Through optimization of static and dynamic parameters,the time of adsorption and desorption equilibrium of cordcepin by the NKA-II resin was 6h and 3h,respectively.The current velocity of adsorption on the column was 1 BV/h,and the eluting reagent was 70% ethanol with 4BV/h eluting rate after eliminating the impurity by 10% ethanol.By combination of those optimum conditions,the content of cordycepin,and the recovery rate achieved at 35% and 90%,respectively.Furthermore,the purity of Cordycepin was 98% through recrystallization for several times.