Fermentation Characters of Fatty Acids Hydroxylation and Optimization of Culture Conditions by Bacillus pumilus

Abstract:

A mutated strain of Bacillus pumilus M-F641 was used for production of ω-hydroxy fatty acids(ω-HFA)by bio-conversion of oleic acid.The optimum carbon source of the medium and fermentation conditions were investigated for the cell growth,NADPH content and conversion yield of oleic acid as index.On the basis of single factor experiment,the optimal fermentation condition was obtained by response surface methodology,using the oleic acid conversion rate as index.Further experiment was conducted under the optimal culture condition.The optimum conditions were listed as follows:the glucose concentration 20 g/L,the environmental conditions were 30℃,pH 7.4 and 60 mL in 250mL flask,respectively.Under the optimum conditions,oleic acid transformation yield was achieved at 74.58%,and the ratio of ω-HFA with total oleic acid was 22.3%.ω-HFA production yield was significantly improved under the optimized fermentation conditions.