Isolation and Identification of a Strain JH-1 Removal Cholesterol

Abstract:

More than 100 bacteria were isolated from the fermented food such as ham,yogurt,and pickle in this study.With Cholesterol-reducing ability inMRS-CHOL medium as index,one strain was screened and named as JH-1.The cholesterol removarate of strain JH-1 is 48.68%.Upon chemotaxonomic characterization and phylogenetic inference based 16S rDNA analysis,the strain JH-1 was identified as Lactobacillus brevis strain.The removal cholesterol ability of Lactobacillus brevis JH-1 was studied.The result listed as follows: the cultivation time is 48h,the inoculation percentage is 3%,the temperature is 37℃,the initial pH of the media is 6 and the cholesterol contents is 1mg/mL.Lactobacillus brevis JH-1 has the best removal cholesterol ability.