Construction and Characterization of Campylobacter jejuni flhA Mutant Strain

Abstract:

flhA,encoded by the gene flhA,is an important component of the flagellar export apparatus of Campylobacter jejuni.Here we constructed an flhA mutant strain HXW001.Compared with that of the wild type,the flhA mutant cell could grow well but lack flagella,the motility of the mutant cells was lost,and the ability of the mutant cells for the formation of biofilm and autoagglutination also decreased.In addition,it was found that the expression of the flagellin gene flaA was affected by flhA.These findings indicated that flhA was the molecular basis of flagellar synthesis and motility of Campylobacter jejuni and that flhA was closely related to pathogenicity of Campylobacter jejuni.