Establishment of a Two-Dimensional Electrophoresis Protocol for Extracellular Enzymes Excreted by Microorganisms from Shaoxing Rice Wine Wheat Qu

Abstract:

In this study,the two-dimensional(2-D) electrophoresis was used to separate the total extracellular enzymes were excreted by the microorganisms of wheat qu of Shaoxing rice wine.A 2-D electrophoresis technique which was suitable for the extracellular enzymes was established through investigating the the isoelectric focusing conditions,pH values of extraction buffer,and sampling volumes and other key factors on the effects of 2-D electrophoresis..It was demonstrated that the 2-D electrophoresis profiles had high resolution and good reproducibility.The results present here provideda basis for further identification of the extracellular enzymes excreted by the microorganisms from Shaoxing rice wine wheat qu by MALDI-TOF-MS/MS analysis.