Preliminary Study on the Anaerobic Biological Hydrolysis of Insoluble Compounds in Cassava Stillage

Abstract:

The aim of this manuscript is to determine the effect of pH on the cassava stillage hydrolyzation at 60℃ through compared with the values of the cellulase(CMCase),hemicellulase(xylase) activity,the free and cell-bound enzyme activities,hydrolytic ratio,the degradation ratio of suspended solids(SS),the total concentration of volatile fatty acids(VFA) and ethanol,the degradation ratio of cellulose and hemicelluloses at different pH conditions.The results showed that higher cellulase and hemicellulase activity(1 588 and 822 IU/L),degradation ratio of SS(37.3%),hydrolytic ratio of cellulose and hemicellulose(54.9% and 58.4%) were obtained when pH kept at 7.0~8.0.Under this condition,the activities of the free and cell-bound enzyme were the same,the total concentration of VFA and ethanol was achieved at 12.6 g/L,where acetate was the main component.