Study on the Interaction with DNA and Antioxidant Activity of the Aspartic Protease Inhibitors from Potato

Abstract:

The purpose of this study was to purify a kind of aspartic protease inhibitor from potato and investigate its interaction with DNA and antioxidant activity.With ethidium bromide as a probe,Fluorescence method for screening anticancer drugs in the application,the first time the interaction between PPI and DNA has been studied;Use scavenging of free radicals of flavonoids,DPPH,H2O2/Fe2+ for hydroxyl radical system and superoxide anion radical system of the PPI in vitro antioxidant activity study,The results show that PPI has some antioxidant activity in vitro and from the point of view with the DNA may have anticancer effects.