Production and Identification of Monoclonal Antibodies to Melamine

Abstract:

In order to establish the immunoassay method of melamine(MEL),two artificial antigens(MEL-BSA,MEL-OVA) were prepared by glutaraldehyde crosslinking method,which were identified by UV absorbency spectrum.MEL-BSA was used as immuneogen to produce a cell strain(6E3) secreting monoclonal antibody against melamine from BALB/c.After purification,its titers of ascetic fluids were 64000 by indirect ELISA.The lineal range and half-inhibitory concentration(IC50) of the monoclonal antibody were from 0.1 to 3.2 mg/L and 1.01 mg/L.The percentages of cross-activity to cyanuric acid,Ammelid and Ammeline were all less than 0.1%.Results indicated that the monoclonal antibody against melamine would be useful in the development of melamine residue ELISA test kit or colloid gold-immunochromatography assay strip.